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. 1999 Dec 21;96(26):15190–15195. doi: 10.1073/pnas.96.26.15190

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Copyright © 1999, The National Academy of Sciences

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BCG reside within vesicles permeable to cytosolic macromolecules of up to 70 kDa in size. Macrophages infected with BCG-GFP and microinjected with 3- or 40-kDa Texas Red-tagged dextrans or tagged-ovalbumin showed appreciable colocalization of the two signals. Microinjection of 70-kDa tagged dextrans into macrophages infected with live BCG-GFP demonstrates reduced colocalization, probably indicating an upper limit of accessibility to the phagosome. Injection of 2,000 kDa failed to reveal any appreciable signal colocalization.