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. 2008 Feb 28;586(Pt 9):2277–2291. doi: 10.1113/jphysiol.2007.149021

Figure 5. preBötC neurons in primary culture.

Figure 5

A, staining for 5-HT-4a receptors (green) and MAP2 (red). B, staining for NK-1 (green) and μ-opioid receptors (red). C, cluster of active respiratory neurons. D, calcium changes. Multicoloured traces correspond to ROIs indicated in C. A sample trial is presented in Supplementary Movie 3. E, calcium transients in the cell soma and membrane current measured at the holding potential −40 mV. Scale bars in all panels, 20 μm.