Localization of leptin receptor mRNA and protein in fetal PAT, liver and skeletal muscle from a saline-infused fetus at 130 days of gestation. A–C, leptin receptor mRNA localization in fetal PAT, liver and skeletal muscle, respectively; D–F, control hybridization with sense probes in fetal PAT, liver and skeletal muscle, respectively; G–I, leptin receptor protein localization in fetal PAT, liver and skeletal muscle, respectively; J–L, negative control for leptin receptor protein in fetal PAT, liver and skeletal muscle, respectively. Scale bars indicate 50 μm.