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. 2008 Jan 31;586(Pt 9):2253–2261. doi: 10.1113/jphysiol.2007.142521

Figure 5. Inhibitory effects of EGTA on the activity-dependent inactivation and facilitation of IpCa.

Figure 5

IpCa was evoked by 1 ms depolarizing command pulses after dialysing calyces with a patch pipette solution containing 10 mm EGTA. A, at P7–9 calyces, EGTA markedly reduced IpCa inactivation induced by the paired-pulse protocol. The maximal IpCa inactivation (at 1 s ISI) was 3.3 ± 0.8% (n = 5), which was significantly less than control in Fig. 1 (P < 0.01). B, at P13–15 calyces, IpCa was evoked by 2 s trains of 1 ms command pulses at 50 Hz, 100 Hz and 200 Hz, with 0.5 mm (black, n = 4) or 10 mm (red, n = 4) EGTA included in the patch pipette solution. On the ordinate, the IpCa amplitude is normalized to the initial one.