A, local unilateral application of ATPA (10 μm, 15 s) into the preBötC in control, immediately after 90 s pre-application of UBP-302 (10 μm) 15 min later, and following 15 min of washout. B, application of 100 μm ATPA into the same site as in A, showing dose dependence of the ATPA-evoked frequency response. C, group data (n = 5) showing relative effects on frequency of applying into the preBötC 100 μm ATPA (15 s) alone, and 10 μm ATPA in control, after 90 s pre-application of 10 μm UBP-302 and after washout of UBP-302 for 15 min † indicates Significant difference from control, P < 0.001; * indicates significant difference from control and 100 μm ATPA, P < 0.001; ** indicates significant difference from control, P < 0.05; § indicates significant difference from ATPA + UBP-302, P < 0.05.