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. 2008 Apr 4;95(3):1412–1427. doi: 10.1529/biophysj.108.130385

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Mobilizing SR Ca²⁺ with caffeine. (A) Rat myocyte, AM-loaded with Fluo-3 and initially paced at 2 Hz in NT was then superfused with 0Na0Ca medium containing 10 μM CPA (stabilizing solution, SS), interspersed with three 30-s episodes of SS + 10 mM caffeine. (Upper panel) time course for F/F₀ averaged along a longitudinal line scan (inset indicates position of line scan axis); (middle panel) F/F₀ line scan; (lower panel) cell shortening. The first caffeine exposure mobilizes all SR Ca²⁺. (B) Different rat myocyte exposed to SS containing 10 mM caffeine and 10 mM [Ca²⁺]_o; note lack of -recovery, suggesting block of sarcolemmal PMCA. Final recovery in 0Na0Ca (with zero CPA) is attributable to SERCA and PMCA activity.

Inline graphic — Mobilizing SR Ca²⁺ with caffeine. (A) Rat myocyte, AM-loaded with Fluo-3 and initially paced at 2 Hz in NT was then superfused with 0Na0Ca medium containing 10 μM CPA (stabilizing solution, SS), interspersed with three 30-s episodes of SS + 10 mM caffeine. (Upper panel) time course for F/F₀ averaged along a longitudinal line scan (inset indicates position of line scan axis); (middle panel) F/F₀ line scan; (lower panel) cell shortening. The first caffeine exposure mobilizes all SR Ca²⁺. (B) Different rat myocyte exposed to SS containing 10 mM caffeine and 10 mM [Ca²⁺]_o; note lack of -recovery, suggesting block of sarcolemmal PMCA. Final recovery in 0Na0Ca (with zero CPA) is attributable to SERCA and PMCA activity.