FIGURE 4.

(A) Normalized crosspeak intensities observed in the constant time HACACO NMR measurements as a function of the residue number. Open circles and solid diamonds represent the values for HPV-16 E2 in absence and presence of DNA, respectively. The residues of the recognition helix α1 that show a significant enhanced crosspeak intensity are labeled. (B) The DNA facing surface of HPV-16 E2 protein. Red and blue indicate the residues having the highest and the lowest crosspeak intensity, respectively. The residues Asn²⁹⁴, Cys²⁹⁸, Tyr³⁰², and Lys³⁰⁵ belonging to the α1-helix and important for the DNA binding, are labeled.