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. 2008 Apr 4;95(3):1360–1370. doi: 10.1529/biophysj.108.130237

FIGURE 4.

FIGURE 4

Mechanical properties of C. elegans twitchin kinase. (A) Two examples of force-extension curves obtained for the Fn-Twc kinase-Ig construct. The two small force peaks correspond to the stepwise unfolding of the Twc kinase domain and the last two peaks to the unfolding of the flanking Ig/Fn domains. (B) Histogram of increases in contour length increments observed on unfolding, ΔL, of Fn-Twc kinase-Ig. There are peaks at ∼30 nm, 65 nm, and 95 nm (Gaussian fits: 31 ± 5 nm, 67 ± 18 nm, and 97 ± 10 nm, n = 142), which correspond to the unfolding of Ig/Fn domains (blue bars), and kinase domain (red and green bars). (C) Unfolding force distributions for Ig/Fn domains (blue bars) and kinase domain (red and green bars); the respective force peaks are at 111 ± 67 pN (n = 57), 83 ± 57 pN (n = 41), and 52 ± 11 pN (n = 43).