Table 1.
Activation of LEE operons by preconditioned media
| lacZ fusions | β-galactosidase activity LB | β-galactosidase activity LB-PC* |
|---|---|---|
| LEE1† | 178 ± 62.12‡ | 523 ± 80 |
| LEE2† | 155 ± 25 | 455 ± 23 |
| LEE3† | 76 ± 17 | 100 ± 27 |
| LEE3 (pSE1093)† | 285 ± 14 | 603 ± 32 |
| tir† | 32 ± 3.4 | 42 ± 3.6 |
| tir (pSE1093)† | 62 ± 3 | 212 ± 79 |
| stx† | 30 ± 7 | 45 ± 6 |
| bla† | 368 ± 48 | 361 ± 37 |
| LEE1 (MC4100)§ | 860 ± 276 | 4,118 ± 230 |
| LEE1 (86-24h11)§ | 1,681 ± 276 | 5,266 ± 230 |
| LEE2 (MC4100)§ | 1,563 ± 153 | 3,113 ± 116 |
| LEE2 (86-24h11)§ | 1,597 ± 239 | 6,525 ± 507 |
| LEE3 (MC4100)§ | 1,078 ± 25 | 1,066 ± 50 |
| LEE3 (86-24h11)§ | 1,074 ± 21 | 2,200 ± 237 |
*
LB-PC, LB medium preconditioned with EHEC strain 86-24h11.
† Single copy lacZ fusions in K-12 strain TE2680; pSE1093 contains EHEC ler gene.
‡ β-galactosidase activity in Miller units.
§ Multicopy lacZ fusions in K-12 strain MC4100 or EHEC strain 86-24h11.