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Fluorescence emission spectra of probe F2N8 in LUVs of different lipid composition. (A) When present, cholesterol (Chol) was added at 35 mol % with respect to phospholipids. In the ternary mixture, the molar ratio of lipids was DOPC/SM/Chol = 1/1/0.7. The spectra were normalized at the T* band maximum. Excitation wavelength was 400 nm. (B and C) Results of the three-band deconvolution of the fluorescence spectra of F2N8 in LUV composed either of SM (B) or SM/Chol (C).
