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Comparison of FTIR difference spectra of phyA reconstituted with nonlabeled PΦB and phyA reconstituted with PΦB in H2O (gray) and D2O (black). The spectra (from top to bottom) refer to the “Pfr” minus “Pr” (A), “lumi-R” minus “Pr” (B), and “lumi-F” minus “Pfr” (C) differences. Protein changes are denoted by an asterisk. Further details are given in the text (Materials and Methods).
