TABLE 1.

Quantification of colloidal bead populations with CLSM

Nominal diameter as stated by manufacturer (nm)	Measured mean diameter, DM (nm)	Gaussian mean diameter, D0 (nm)	Gaussian halfwidth, w (nm)	Mean diameter displacement, ΔD0 (nm)
100 ± 6	123	113 ± 1	17 ± 0	0
210 ± 21	209	178 ± 1	21 ± 1	4
500 ± 20	515	521 ± 2	53 ± 3	37
1000 ± 32	977	1002 ± 6	99 ± 7	243

Comparison of labeled colloidal bead specifications with measured values. From the left, the first column displays the nominal bead diameter and intersample variations of the mean as stated by the manufacturer. Columns 2–5 show measured mean diameter, D_M, Gaussian mean diameter, D₀, Gaussian halfwidth of population, w, and mean diameter displacement, ΔD₀. The quantities D₀, w, and ΔD₀ are quantities resulting from the Gaussian fit of the populations in Fig. 3 B, whereas D_M is the mean value of all converted beads. The mean diameter displacement, ΔD₀,_, is calculated as the difference between homogeneous and confocal illumination graphs in Fig. 3 A.