TABLE 2.

Characterization of an extruded vesicle sample (SUV₂₀₀) with CLSM, cryoTEM, and DLS

Method	Mean diameter of distribution (nm)	SD of mean diameter (nm)	SD of population (polydispersity) (nm)
CLSM	106	12	61
CryoTEM	114	23	50
DLS	116	7	80

The size distribution of vesicles extruded at 200 nm was determined using the CLSM model and compared to the cryoTEM and DLS models. Standard deviation (SD) of the mean diameter was calculated in the case of CLSM and cryoTEM using several individual micrographs from each sample. In the case of DLS, the standard deviation of the mean was obtained from the average value from 10 measurements.