Table 1.
Proteins identified from both cell lines, TOV112D, and MDAH2774.
| Access No. | Protein Name | Expression change(fold)Theritical Experimental pI | ||||
|---|---|---|---|---|---|---|
| TOV112D | MDAH2774 | pI | TOV 112D | MDAH 2774 | ||
| P31947 | 14-3-3 protein sigma | 98.4 | 4.68 | pH4.6-4.4 | pH4.4-4.2 | |
| P62736 | Actin, aortic smooth muscle | 6.3 | 5.24 | pH5.6-5.4 | pH5.2-5.0 | |
| P60709 | Actin, cytoplasmic 1 | 3.6 | 5.29 | pH5.6-5.4 | pH5.2-5.0 | |
| P63261 | Actin, cytoplasmic 2 (Gamma-actin) | — | — | 5.31 | abovepH7.5 | abovepH7.5 |
| P12814 | Alpha-actinin-1 (Alpha-actinin cytoskeletal isoform) | 1.1 | 5.25 | pH5.2-5.0 | pH4.2-4.0 | |
| O43707 | Alpha-actinin-4 (Non-muscle alpha-actinin 4) | — | — | 5.27 | abovepH7.5 | above pH 7.5 |
| Q9H2P0 | Activity-dependent neuroprotector | 3.1 | 6.97 | pH6.2-6.0 | pH6.8-6.6 | |
| P51825 | AF4/FMR2 family member 1,Proto-oncogene AF4 | 2.7 | 9.26 | abovepH7.5 | abovepH7.5 | |
| Q16352 | Alpha-internexin | — | — | 5.34 | abovepH7.5 | salt washing |
| P02768 | Serum albumin [Precursor] | 8.9 | 5.67 | pH5.6-5.4 | pH5.6-5.4 | |
| Q01484 | Ankyrin-2 | — | — | 5.03 | pH5.0-4.8 | pH5.2-5.0 |
| Q86YR6 | Ankyrin repeat domain-containing protein 21 | 2.6 | 6.32 | pH6.6-6.4 | pH7.0-6.8 | |
| P50995 | Annexin A11 (Annexin XI) | — | — | 7.53 | salt washing | salt washing |
| P07355 | Annexin A2 | 7.2 | 7.56 | pH5.8-5.6 | pH6.0-5.8 | |
| P12429 | Annexin A3 | 8.8 | 5.63 | pH5.6-5.4 | pH5.6-5.4 | |
| Q9NR81 | Rho guanine nucleotide exchange factor 3 | 4.4 | 6.03 | pH4.8-4.6 | pH4.4-4.2 | |
| Q68CP9 | AT-rich interactive domain-containing protein 2 | 3.2 | 7.08 | pH7.2-7.0 | pH7.2-7.0 | |
| P06576 | ATP synthase subunit beta, mitochondrial precursor | 15.5 | 5 | pH5.6-5.4 | pH5.6-5.4 | |
| O75173 | ADAMTS-4 [Precursor] | 3.2 | 8.23 | pH4.6-4.4 | abovepH7.5 | |
| Q8WWM7 | Ataxin-2-like protein | 2.7 | 8.7 | above pH7.5 | abovepH7.5 | |
| Q8IY92 | BTB/POZ domain-containing protein 12 | 1.5 | 5.32 | pH6.2-6.0 | abovepH7.5 | |
| Q05682 | Caldesmon (CDM) | 1.8 | 5.63 | abovepH7.5 | abovepH7.5 | |
| P62158 | Calmodulin (CaM) | 6.7 | 4.09 | pH4.4-4.2 | pH4.4-4.2 | |
| P83916 | Chromobox protein homolog 1 | — | — | 4.85 | above pH7.5 | abovepH7.5 |
| P47902 | Homeobox protein CDX-1 | 3.3 | 9.58 | pH6.2-6.0 | pH6.0-5.8 | |
| P49454 | Centromere protein F | 1.7 | 5.03 | pH4.8-4.6 | pH4.6-4.4 | |
| Q86VQ0 | Uncharacterized protein C6orf152 | 1.7 | 7.31 | pH6.6-6.4 | pH6.6-6.4 | |
| Q9HAC7 | Uncharacterized protein C7orf10 | 2.6 | 8.54 | salt washing | salt washing | |
| Q9P2M7 | Cingulin | 2.2 | 5.46 | pH7.2-7.0 | pH7.2-7.0 | |
| P53618 | Coatomer subunit beta | 4.3 | 5.72 | pH4.8-4.6 | pH4.6-4.4 | |
| Q92828 | WD repeat-containing protein 2 | 2 | 8.24 | pH5.6-5.4 | pH6.0-5.8 | |
| Q8IVV8 | Protein FAM77A | 3.2 | 5.43 | above pH7.5 | abovepH7.5 | |
| Q14118 | Dystrophin-associated glycoprotein 1 | 2.4 | 9.31 | above pH7.5 | above pH7.5 | |
| Q9UIK4 | Death-associated protein kinase 2 | 28.5 | 6.45 | pH6.8-6.6 | abovepH7.5 | |
| P16989 | DNA-binding protein A | 27 | 9.77 | above pH7.5 | pH7.2-7.0 | |
| Q13561 | Dynactin subunit 2 (Dynactin complex 50 kDa subunit) | 11.9 | 5.1 | pH5.2-5.0 | pH4.6-4.4 | |
| P17661 | Desmin | 2.6 | 5.21 | pH5.4-5.2 | pH5.4-5.2 | |
| O00273 | DNA fragmentation factor subunit alpha | 1.4 | 4.68 | pH4.8-4.6 | PH4.8-4.6 | |
| O60469 | Down syndrome cell adhesion molecule [Precursor] | 1.7 | 7.81 | pH5.2-5.0 | pH5.2-5.0 | |
| Q8TE73 | Ciliary dynein heavy chain 5 | 2.4 | 5.79 | pH4.8-4.6 | pH4.8-4.6 | |
| Q8WXU2 | Dyslexia susceptibility 1 candidate gene 1 protein | 1.2 | 8.95 | pH5.2-5.0 | pH5.2-5.0 | |
| P68104 | Elongation factor 1-alpha 1 | 35.8 | 9.1 | pH7.6-7.4 | pH7.6-7.4 | |
| P26641 | Elongation factor 1-gamma (EF-1-gamma) | 1 | 6.27 | pH5.2-5.0 | pH4.6-4.4 | |
| P13639 | Elongation factor 2 | 3.2 | 6.42 | 6.2-6.0 | pH6.0-5.8 | |
| Q14677 | Clathrin interactor 1 (Epsin-4) (Epsin-related protein) | 1.7 | 6.01 | pH6.2-6.0 | pH6.2-6.0 | |
| P15311 | Ezrin (p81) (Cytovillin) (Villin-2) | 4.7 | 5.95 | pH6.0-5.8 | pH6.0-5.8 | |
| Q5JRC9 | Protein FAM47A | 2.6 | 9.15 | above pH7.5 | above pH7.5 | |
| Q96AY3 | FK506-binding protein 10 [Precursor] | 3.8 | 5.26 | pH5.4-5.2 | pH5.4-5.2 | |
| Q16595 | Frataxin, mitochondrial [Precursor] | 6.8 | 5.94 | pH4.8-4.6 | pH4.6-4.4 | |
| Q5H8C1 | FRAS1-related extracellular matrix protein 1 [Precursor] | 2.7 | 5.5 | pH4.6-4.4 | salt washing | |
| P32455 | Interferon-induced guanylate-binding protein 1 | 1.2 | 5.97 | pH7.6-7.4 | pH7.2-7.0 | |
| Q8IWJ2 | GRIP and coiled-coil domain-containing protein 2 | 1.3 | 5.08 | above pH7.5 | abovepH7.5 | |
| Q02153 | Guanylate cyclase soluble subunit beta-1 (GCS-beta-1) | 3.7 | 5.2 | salt washing | pH5.4-5.2 | |
| Q96N19 | Integral membrane protein GPR137 | 2.5 | 8.78 | salt washing | pH4.8-4.6 | |
| P38646 | Stress-70 protein, mitochondrial [Precursor] | 150.7 | 5.44 | pH5.6-5.4 | pH5.6-5.4 | |
| P11021 | 78 kDa glucose-regulated protein precursor | 2.7 | 5.01 | pH5.2-5.0 | pH5.2-5.0 | |
| P28001 | Histone H2A type 1-E | 244 | 11.05 | pH6.8-6.6 | pH7.6-7.4 | |
| P0C0S8 | Histone H2A type 1 | 531.9 | 10.9 | above 7.5 | pH7.2-7.0 | |
| P62807 | Histone H2B type 1-C/E/F/G/I | 100 | 10.32 | pH7.6-7.4 | pH7.6-7.4 | |
| P62805 | Histone H4 | 46.2 | 11.36 | pH7.6-7.4 | pH7.6-7.4 | |
| P82979 | Nuclear protein Hcc-1 | 2.6 | 6.12 | pH4.6-4.4 | pH4.6-4.4 | |
| Q16836 | Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial | 1.1 | 8.38 | pH7.4-7.2 | pH7.0-6.8 | |
| Q8TBE9 | N-acylneuraminate-9-phosphatase(NANP_human) | 9.7 | 6.01 | pH4.6-4.4 | pH6.2-6.0 | |
| P31943 | Heterogeneous nuclear ribonucleoprotein H (hnRNP H) | 58.6 | 5.89 | pH5.8-5.6 | pH5.6-5.4 | |
| P52597 | Heterogeneous nuclear ribonucleoprotein F (hnRNP F) | 2 | 5.38 | pH5.6-5.4 | pH5.6-5.4 | |
| P61978 | Heterogeneous nuclear ribonucleoprotein K (hnRNP K) | 1.8 | 5.39 | pH5.2-5.0 | pH4.8-4.6 | |
| O43390 | Heterogeneous nuclear ribonucleoprotein R (hnRNP R) | 1.1 | 8.23 | salt washing | salt washing | |
| P34931 | Heat shock 70 kDa protein 1L | 15.3 | 5.76 | pH5.8-5.6 | pH5.6-5.4 | |
| P07900 | Heat shock protein HSP 90-alpha | 13.8 | 4.94 | pH6.6-6.4 | pH4.4-4.2 | |
| P08107 | Heat shock 70 kDa protein 1 | 7.7 | 5.48 | pH5.4-5.2 | pH5.2-5.0 | |
| P54652 | Heat shock-related 70 kDa protein 2 | 6 | 5.56 | pH5.6-5.4 | pH5.6-5.4 | |
| P34932 | Heat shock 70 kDa protein 4 | 1.2 | 5.18 | pH5.2-5.0 | pH4.8-4.6 | |
| P11142 | Heat shock cognate 71 kDa protein | 7.8 | 5.37 | pH5.6-5.4 | pH5.6-5.4 | |
| Q9Y3Y2 | Uncharacterized protein C1orf77 | 1.6 | 12.24 | above pH7.5 | 7.6-7.4 | |
| Q7Z6Z7 | E3 ubiquitin-protein ligase HUWE1 | 2.2 | 5.1 | above pH7.5 | above pH7.5 | |
| P20810 | Calpastatin | 4.5 | 4.98 | above pH7.5 | above pH7.5 | |
| P63241 | Eukaryotic translation initiation factor 5A-1 | 1.4 | 5.08 | pH5.0-4.8 | pH5.0-4.8 | |
| P01563 | Interferon alpha-2 [Precursor] | 1.3 | 5.99 | pH6.0-5.8 | pH6.0-5.8 | |
| Q12906 | Interleukin enhancer-binding factor 3 | 1.3 | 8.86 | salt washing | pH5.2-5.0 | |
| Q14643 | Inositol 1,4,5-trisphosphate receptor type 1 | 2.3 | 5.71 | pH5.8-5.6 | pH5.8-5.6 | |
| P29375 | Histone demethylase JARID1A | 1.1 | 6.42 | above pH7.5 | pH7.0-6.8 | |
| O94953 | JmjC domain-containing histone demethylation protein 3B | 34.1 | 6.72 | pH6.8-6.6 | above pH7.5 | |
| Q9HCJ3 | Ribonucleoprotein PTB-binding 2 | 1.4 | 7.12 | pH7.0-6.8 | above pH7.5 | |
| P13645 | Keratin, type I cytoskeletal 10 | 34 | 5.13 | pH5.8-5.6 | pH6.0-5.8 | |
| P13646 | Keratin, type I cytoskeletal 13 | 3.6 | 4.91 | pH7.6-7.4 | pH7.6-7.4 | |
| P08779 | Keratin, type I cytoskeletal 16 | 3.7 | 4.98 | pH7.6-7.4 | pH5.2-5.0 | |
| P12035 | Keratin, type II cytoskeletal 3 | 2.1 | 8.16 | pH5.6-5.4 | pH5.6-5.4 | |
| P05787 | Keratin, type II cytoskeletal 8 | 30 | 5.52 | pH5.6-5.4 | pH5.6-5.4 | |
| P00568 | Adenylate kinase isoenzyme 1 | 11.1 | 8.73 | above 7.5 | pH7.2-7.0 | |
| O95069 | Potassium channel subfamily K member 2 | 1.4 | 8.46 | above pH7.5 | pH7.2-7.0 | |
| Q9NQT8 | Kinesin-like protein KIF13B | 3.4 | 5.56 | pH7.2-7.0 | above pH7.5 | |
| P46013 | Antigen KI-67 | 1.1 | 9.49 | above pH7.5 | pH6.6-6.4 | |
| O00139 | Kinesin-like protein KIF2A | 6.8 | 6.04 | pH6.2-6.0 | above pH7.5 | |
| Q9BVG8 | Kinesin-like protein KIFC3 | 4.3 | 7.62 | pH7.4-7.2 | pH7.2-7.0 | |
| P05455 | Lupus La protein | 2.3 | 6.68 | pH6.0-5.8 | pH5.6-5.4 | |
| P20700 | Lamin-B1 | 5.4 | 5.11 | pH5.2-5.0 | pH5.0-4.8 | |
| P02545 | Lamin-A/C | 16.3 | 6.57 | pH6.2-6.0 | pH5.6-5.4 | |
| Q14847 | LIM and SH3 domain protein 1 | 1.6 | 6.61 | pH6.8-6.6 | pH4.6-4.4 | |
| Q6JVE6 | Epididymal-specific lipocalin-10 [Precursor] | 1.5 | 10.25 | above pH7.5 | above pH7.5 | |
| P07195 | L-lactate dehydrogenase B chain | 4.3 | 5.72 | pH6.2-6.0 | pH5.6-5.4 | |
| Q7L1W4 | Leucine-rich repeat-containing protein 8D | 1.5 | 7.76 | 7.6-7.4 | pH5.2-5.0 | |
| Q9NX58 | Cell growth-regulating nucleolar protein | 1.3 | 9.57 | pH4.8-4.6 | pH6.0-5.8 | |
| Q9UPN3 | Microtubule-actin cross-linking factor 1, isoforms 1/2/3/5 | 1.6 | 5.27 | pH7.0-6.8 | above pH7.5 | |
| P27816 | Microtubule-associated protein 4 | 1 | 5.32 | pH5.0-4.8 | pH4.6-4.4 | |
| P43243 | Matrin-3 | 2.8 | 5.87 | pH4.8-4.6 | pH4.8-4.6 | |
| P41594 | Metabotropic glutamate receptor 5 [Precursor] | 2.9 | 8.09 | pH5.8-5.6 | pH5.6-5.4 | |
| P19105 | Myosin regulatory light chain 2, nonsarcomeric | 2.3 | 4.67 | pH4.8-4.6 | pH4.8-4.6 | |
| P49006 | MARCKS-related protein | 4 | 4.68 | pH4.6-4.4 | pH4.6-4.4 | |
| P60660 | Myosin light polypeptide 6 | 22.2 | 4.56 | above pH7.5 | pH4.8-4.6 | |
| Q15746 | Myosin light chain kinase, smooth muscle | — | — | 5.85 | pH6.20-6.0 | above pH7.5 |
| O95251 | Histone acetyltransferase MYST2 | 1.4 | 9.01 | pH4.8-4.6 | pH7.0-6.8 | |
| P15531 | Nucleoside diphosphate kinase A | 3 | 5.83 | pH5.6-5.4 | pH5.2-5.0 | |
| P20929 | Nebulin | 1.4 | 9.1 | salt washing | pH5.2-5.0 | |
| P82970 | Nucleosome-binding protein 1 | 1.8 | 4.5 | pH4.4-4.2 | pH4.4-4.2 | |
| Q9UNZ2 | NSFL1 cofactor p47 | 1.2 | 4.99 | pH4.6-4.4 | pH4.6-4.4 | |
| Q02818 | Nucleobindin-1 [Precursor] | 6 | 5.09 | pH5.2-5.0 | pH5.2-5.0 | |
| Q96KK4 | Olfactory receptor 10C1 | — | — | 8.64 | above pH7.5 | above pH7.5 |
| Q9Y4L1 | Hypoxia up-regulated protein 1 [Precursor] | 1.2 | 5.07 | pH5.0-4.8 | pH4.8-4.6 | |
| Q9Y6V0 | Protein piccolo | 1.4 | 6.12 | pH6.2-6.0 | pH6.2-6.0 | |
| O95831 | Apoptosis-inducing factor 1, mitochondrial [Precursor] | 5.5 | 6.86 | pH6.2-6.0 | pH5.2-5.0 | |
| P30101 | Protein disulfide-isomerase A3 [Precursor] | 6.9 | 5.61 | pH5.6-5.4 | pH5.6-5.4 | |
| P41219 | Peripherin | 1.6 | 5.37 | above pH7.5 | above pH7.5 | |
| O43933 | Peroxisome biogenesis factor 1 | — | — | 5.91 | pH4.6-4.4 | pH7.2-7.0 |
| Q9NQP4 | Prefoldin subunit 4 | 3 | 4.42 | pH4.4-4.2 | pH4.4-4.2 | |
| P78562 | Phosphate-regulating neutral endopeptidase | 1.8 | 8.91 | salt washing | pH5.2-5.0 | |
| Q9ULU4 | Protein kinase C-binding protein 1 | 1.8 | 6.83 | 7.4-7.2 | pH7.2-7.0 | |
| Q96MT3 | Prickle-like protein 1 | 4.1 | 5.84 | pH5.2-5.0 | pH5.2-5.0 | |
| P17980 | 26S protease regulatory subunit 6A | 13.3 | 5.13 | pH5.2-5.0 | pH7.6-7.4 | |
| Q15678 | Tyrosine-protein phosphatase non-receptor type 14 | — | — | 8.45 | above pH7.5 | above pH7.5 |
| P11498 | Pyruvate carboxylase, mitochondrial [Precursor] | 1.1 | 6.14 | pH6.8-6.6 | pH7.0-6.8 | |
| Q7Z5J4 | Retinoic acid-induced protein 1 | 16.5 | 9.03 | pH5.6-5.4 | pH7.2-7.0 | |
| P43487 | Ran-specific GTPase-activating protein | 4.6 | 5.19 | pH5.2-5.0 | pH5.0-4.8 | |
| O76081 | Regulator of G-protein signaling 20 | 5.4 | 6.48 | pH6.6-6.4 | pH6.6-6.4 | |
| Q99729 | Heterogeneous nuclear ribonucleoprotein A/B | 1.5 | 9.04 | above pH7.5 | ph4.8-4.6 | |
| P62263 | 40S ribosomal protein S14 | 1.3 | 10.08 | pH6.2-6.0 | pH7.6-7.4 | |
| Q92545 | Transmembrane protein 131 [Fragment] | 1.8 | no info | pH7.2-7.0 | abovepH7.5 | |
| Q15413 | Ryanodine receptor 3 | — | — | 5.45 | pH7.2-7.0 | abovepH7.5 |
| Q92562 | SAC domain-containing protein 3 | 1.5 | 6.46 | pH7.2-7.0 | above pH7.5 | |
| Q9Y5Y9 | Sodium channel protein type 10 subunit alpha | 2.8 | 5.67 | pH6.4-6.2 | pH6.4-6.2 | |
| Q9H190 | Syntenin-2 | 17.8 | 9.15 | pH7.6-7.4 | pH4.0-3.8 | |
| O43175 | D-3-phosphoglycerate dehydrogenase | 159.5 | 6.31 | pH6.6-6.4 | pH5.2-5.0 | |
| O15047 | Histone-lysine N-methyltransferase | 1.5 | 5.07 | pH5.2-5.0 | pH5.2-5.0 | |
| P23246 | Splicing factor, proline- and glutamine-rich | 1.3 | 9.45 | pH6.4-6.2 | pH6.2-6.0 | |
| P84103 | Splicing factor, arginine/serine-rich 3 | 1.1 | 11.64 | above pH7.5 | pH5.2-5.0 | |
| Q13813 | Spectrin alpha chain, brain | 7 | 5.22 | pH5.0-4.8 | pH5.4-5.2 | |
| Q01082 | Spectrin beta chain, brain 1 | 4 | 5.39 | pH5.0-4.8 | pH6.2-6.0 | |
| Q13748 | Tubulin alpha-2 chain | 2 | 4.98 | pH5.2-5.0 | pH5.0-4.8 | |
| Q71U36 | Tubulin alpha-1A chain | 2.7 | 4.94 | pH5.0-4.8 | pH5.0-4.8 | |
| O75764 | Transcription elongation factor A protein 3 | — | — | 9.32 | abovepH7.5 | pH5.6-5.4 |
| P48643 | T-complex protein 1 subunit epsilon | 1.7 | 5.45 | pH5.6-5.4 | pH5.2-5.0 | |
| P49368 | T-complex protein 1 subunit gamma | 1.2 | 6.1 | pH4.6-4.4 | pH5.6-5.4 | |
| Q9UNS1 | Timeless homolog | 2.3 | 5.26 | pH5.6-5.4 | pH5.6-5.4 | |
| P11388 | DNA topoisomerase 2-alpha | 1.4 | 8.82 | above pH7.5 | pH7.2-7.0 | |
| P55327 | Tumor protein D52 | 1.1 | 4.94 | pH5.0-4.8 | pH4.8-4.6 | |
| O43399 | Tumor protein D54 | 3 | 5.26 | pH5.0-4.8 | pH5.0-4.8 | |
| P09493 | Tropomyosin-1 alpha chain | 1.6 | 4.69 | pH4.4-4.2 | pH4.4-4.2 | |
| P07951 | Tropomyosin beta chain | 21.7 | 4.66 | pH4.8-4.6 | pH4.8-4.6 | |
| P06753 | Tropomyosin alpha-3 chain | 1.4 | 4.68 | pH4.8-4.6 | pH4.8-4.6 | |
| Q9Y4A5 | Transformation/transcription domain-associated protein | 1.4 | 8.49 | above pH7.5 | above pH7.5 | |
| P26368 | Splicing factor U2AF 65 kDa subunit | 1.5 | 9.19 | salt washing | pH4.8-4.6 | |
| Q00341 | Vigilin | 3.2 | 6.43 | pH6.6-6.4 | pH 6.6-6.4 | |
| P08670 | Vimentin | 1.3 | 5.06 | pH4.8-4.6 | pH4.8-4.6 | |
| P18206 | Vinculin | 8.3 | 5.51 | pH5.2-5.0 | pH5.6-5.4 | |
| Q9P1Q0 | Vacuolar protein sorting-associated protein 54 | 2.7 | 6.1 | pH5.6-5.4 | pH5.2-5.0 | |
| P04275 | Von Willebrand factor [Precursor] | 2.6 | 5.06 | pH5.2-5.0 | pH5.2-5.0 | |
| Q9P2L0 | WD repeat protein 35 | 1.1 | 5.98 | pH5.2-5.0 | pH4.2-4.0 | |
| Q8NI36 | WD repeat protein 36 | 21.6 | 7.33 | pH7.4-7.2 | abovepH7.5 | |
| O15213 | WD repeat protein 46 | 23.7 | 9.69 | pH7.6-7.4 | abovepH7.5 | |
| Q8IZP6 | RING finger protein 113B | 1.1 | 7.54 | pH5.6-5.2 | pH7.6-7.4 | |
| Q5TAX3 | Zinc finger CCHC domain-containing protein 11 | 1.2 | 8.3 | pH7.0-6.8 | pH7.6-7.4 | |
| Q9H4I2 | Zinc fingers and homeoboxes protein 3 | 2.5 | 5.73 | pH7.6-7.4 | pH7.0-6.8 | |
| Q9UL59 | Zinc finger protein 214 | 3.7 | 8.89 | pH7.6-7.4 | pH7.2-7.0 | |
| O15015 | Zinc finger protein 646 | 1.1 | 6.97 | pH7.0-6.8 | above pH7.5 | |