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Fluorescence outliers
| Preparation | No. | Probe | Events, %* |
|---|---|---|---|
| Beads | 7 | Rhodamine-like | 0.15 ± 0.01 |
| Synaptic boutons | 9 | Rhodamine 123 | 0.29 ± 0.03 |
| Synaptic boutons | 9 | Rhod-2 AM | 1.09 ± 0.1 |
| Synaptic boutons | 7 | Fluo-3 AM | 1.47 ± 0.06 |
| Hippocampal cell, normal medium | 9 | Fluo-3 AM | 0.9 ± 0.05 |
| Hippocampal cell, nominally calcium-free medium | 6 | Fluo-3 AM | 1.08 ± 0.09 |
*Data are shown as means ± SEM.
