Figure 5.

Reduced glial activation in APP^V717F+/− Apoe^−/− TG mice. Glial activation was evaluated by GFAP immunohistochemistry (A and B) and tomato lectin histochemistry (C and D) on paraffin-embedded brain sections from 21/22-mo-old APP^V717F+/− Apoe^+/+ and APP^V717F+/− Apoe^−/− mice (see Materials and Methods for details). A range of glial staining for each genotype analyzed was apparent with an increase in GFAP immunoreactivity and lectin staining in the superficial layers of the cerebral cortex and hippocampus in APP^V717F+/− Apoe^+/+ mice (A and C, respectively) compared with APP^V717F+/− Apoe^−/− mice (B and D, respectively). Note clusters of intensely stained astrocytes (A, Inset) and microglia (C, Inset), which are more prominent in APP^V717F+/− Apoe^+/+ than in APP^V717F+/− Apoe^−/− mice. (×5; Inset, ×40.)