Table 1.

The effects of purine and pyrimidine nucleotides and non-hydrolyzable nucleotide analogs on DNA synthesis in VVEC, AOEC, and MPAEC

Agonist (100 μM)	VVEC	AOEC	MPAEC
Basal	100	100	100
ATP	560 ± 112**	112 ± 1	116 ± 10
ADP	605 ± 74**	143 ± 7	118 ± 8
AMP	488 ± 72*	141 ± 8	112 ± 16
Ado	249 ± 45*	100 ± 8	96 ± 44
ATPγS	335 ± 72*	140 ± 10	122 ± 8
ADPβS	308 ± 117*	141 ± 11	114 ± 12
UTP	150 ± 27	109 ± 15	80 ± 14
UDP	97 ± 4	100 ± 2	79 ± 11
MeSATP	290 ± 44*	132 ± 8	111 ± 12
MeSADP	564 ± 257**	ND	102 ± 10
αβMeATP	141 ± 36	129 ± 10	114 ± 9
βγMeATP	253 ± 121	126 ± 8	105 ± 6
BzATP	200 ± 85	126 ± 18	91 ± 14
Ap4A	198 ± 58	112 ± 3	111 ± 7

The effect of various nucleotides and nucleotide analogs was evaluated by [methyl-³H] thymidine incorporation as described in MATERIALS AND METHODS. Data represents means ± SE (as % of nonstimulated control) from three to five independent experiments, conducted on at least four distinct VVEC populations and at least two AOEC and MPAEC populations; *P < 0.05, **P < 0.01 vs. nonstimulated control