Table 5.
Element concentrations in different subcellular locations in mycorrhizal and non-mycorrhizal root tips of Douglas fir (Pseudotsuga menziesii) variety menziesii (DFM) and variety glauca (DFG)
| Sulfur | Potassium | Magnesium | Iron | ||||||
|---|---|---|---|---|---|---|---|---|---|
| DFM | DFG | DFM | DFG | DFM | DFG | DFM | DFG | ||
| Non-inoculated | Epidermis CW | 18.1 ± 1.3 | 19.0 ± 1.8 | 17.6 ± 8.5 | 15.2 ± 3.0 | 26.6 ± 2.0 | 31.4 ± 7.8 | 4.8 ± 1.4 | 8.0 ± 2.4 |
| Epidermis vacuole | 28.3 ± 2.2 | 13.9 ± 7.5 | 216.5 ± 100.2 | 7.2 ± 2.6 | 21.5 ± 4.0 | 19.6 ± 9.0 | 4.5 ± 1.2 | 5.8 ± 2.2 | |
| Cortex CW | 16.7 ± 1.9 | 18.1 ± 3.9 | 154.6 ± 47.0 | 64.7 ± 30.0 | 10.5 ± 2.4 | 20.4 ± 6.9 | 4.6 ± 1.9 | 6.4 ± 1.2 | |
| Cortex vacuole | 65.2 ± 39.4 | 3.5 ± 1.8 | 188.4 ± 44.0 | 110.7 ± 42.8 | 15.9 ± 3.7 | 15.7 ± 9.5 | 6.3 ± 0.8 | 2.3 ± 0.4 | |
| Central cylinder | 8.3 ± 3.2 | 19.2 ± 3.0 | 36.1 ± 27.2 | 79.0 ± 16.8 | 7.8 ± 2.2 | 17.3 ± 2.5 | 3.2 ± 1.3 | 2.6 ± 0.2 | |
| Rhizopogon | Hyphae mantel | 57.8 ± 6.5 | 37.7 ± 3.6 | 27.3 ± 20.5 | 98.2 ± 58.4 | 37.6 ± 3.1 | 28.8 ± 3.7 | 16.3 ± 7.0 | 8.2 ± 3.0 |
| Hartig net | 44.9 ± 4.8 | 47.1 ± 5.9 | 14.9 ± 6.3 | 115.3 ± 72.2 | 30.1 ± 8.2 | 37.0 ± 6.4 | 9.8 ± 3.3 | 8.7 ± 2.7 | |
| Epidermis CW | 19.6 ± 5.8 | 22.0 ± 1.6 | 28.3 ± 20.6 | 126.5 ± 82.9 | 23.9 ± 11.9 | 34.9 ± 0.2 | 6.4 ± 2.4 | 4.6 ± 1.4 | |
| Epidermis vacuole | 38.1 ± 7.8 | 29.1 ± 8.8 | 91.3 ± 87.2 | 106.3 ± 79.5 | 21.4 ± 3.7 | 24.1 ± 4.1 | 16.6 ± 7.4 | 5.8 ± 0.3 | |
| Cortex CW | 32.1 ± 1.9 | 17.6 ± 10.0 | 126.4 ± 82.6 | 23.5 ± 13.8 | 17.2 ± 7.4 | 22.7 ± 12.4 | 5.4 ± 2.2 | 6.7 ± 3.2 | |
| Cortex vacuole | 40.0 ± 11.1 | 14.7 ± 12.0 | 103.2 ± 59.4 | 138.4 ± 24.4 | 6.6 ± 3.7 | 24.5 ± 12.2 | 6.5 ± 2.5 | 4.0 ± 1.9 | |
| Central cylinder | 13.8 ± 2.9 | 17.6 ± 14.4 | 57.0 ± 28.3 | 99.4 ± 41.6 | 6.6 ± 3.4 | 16.3 ± 2.0 | 5.1 ± 2.0 | 3.5 ± 0.0 | |
| Non-inoculated + Mn | Epidermis CW | 42.2 ± 12.4 | 23.1 ± 6.3 | 36.0 ± 22.3 | 38.9 ± 16.1 | 19.1 ± 7.8 | 18.4 ± 4.9 | 5.8 ± 2.5 | 25.3 ± 17.7 |
| Epidermis vacuole | 42.5 ± 18.7 | 49.9 ± 26.4 | 71.7 ± 44.5 | 338.7 ± 333.2 | 17.4 ± 7.0 | 21.9 ± 7.1 | 3.7 ± 1.9 | 14.1 ± 8.6 | |
| Cortex CW | 54.6 ± 24.1 | 34.3 ± 7.5 | 174.3 ± 96.0 | 110.8 ± 80.5 | 18.2 ± 6.9 | 17.2 ± 4.4 | 7.6 ± 5.3 | 4.2 ± 1.1 | |
| Cortex vacuole | 32.4 ± 6.7 | 56.0 ± 34.3 | 96.2 ± 28.6 | 102.6 ± 82.2 | 6.0 ± 1.7 | 11.7 ± 2.9 | 2.9 ± 0.9 | 5.6 ± 1.0 | |
| Central cylinder | 34.9 ± 12.5 | 26.7 ± 4.1 | 111.0 ± 52.6 | 17.2 ± 9.7 | 11.9 ± 3.1 | 9.1 ± 0.8 | 1.9 ± 0.4 | 9.5 ± 5.9 | |
| Rhizopogon + Mn | Hyphae mantel | 35.7 ± 3.8 | 44.5 ± 15.2 | 10.3 ± 1.5 | 19.1 ± 12.2 | 19.9 ± 7.6 | 29.2 ± 11.8 | 13.6 ± 3.7 | 15.6 ± 4.7 |
| Hartig net | 38.0 ± 5.0 | 42.7 ± 18.0 | 12.5 ± 3.5 | 26.7 ± 19.6 | 36.0 ± 8.1 | 26.1 ± 11.9 | 31.8 ± 20.2 | 16.0 ± 7.8 | |
| Epidermis CW | 24.5 ± 7.3 | 22.0 ± 3.0 | 20.1 ± 10.2 | 13.9 ± 4.1 | 29.7 ± 10.7 | 16.5 ± 3.1 | 14.3 ± 1.7 | 7.7 ± 1.0 | |
| Epidermis vacuole | 25.9 ± 7.4 | 42.5 ± 13.0 | 22.8 ± 18.1 | 7.5 ± 2.5 | 17.9 ± 5.6 | 26.8 ± 8.2 | 5.8 ± 0.9 | 10.3 ± 1.8 | |
| Cortex CW | 24.3 ± 10.3 | 23.7 ± 4.7 | 90.9 ± 31.8 | 24.1 ± 8.7 | 24.3 ± 8.8 | 17.8 ± 4.5 | 17.2 ± 11.0 | 7.9 ± 2.0 | |
| Cortex vacuole | 14.9 ± 8.9 | 45.0 ± 14.0 | 132.1 ± 2.1 | 76.7 ± 33.9 | 8.2 ± 2.2 | 29.0 ± 11.7 | 2.7 ± 0.6 | 4.6 ± 1.7 | |
| Central cylinder | 35.1 ± 19.4 | 74.3 ± 26.6 | 121.0 ± 66.0 | 69.3 ± 27.9 | 12.3 ± 7.0 | 23.6 ± 10.4 | 4.2 ± 2.2 | 4.3 ± 1.1 | |
| Statistic—main effect | |||||||||
| Variety | 0.7021 | 0.7091 | 0.1408 | 0.8748 | |||||
| Main effects | |||||||||
| A: Cell type | 0.5764 | 0.6418 | 0.0402 | 0.7936 | 0.0105 | 0.5410 | 0.2158 | 0.3168 | |
| B: Rhizopogon | 0.2372 | 0.5051 | 0.2069 | 0.6381 | 0.6694 | 0.1240 | 0.0225 | 0.3243 | |
| C: Mn stress | 0.4203 | 0.0020 | 0.5555 | 0.9460 | 0.8230 | 0.3062 | 0.8732 | 0.0800 | |
| Interactions | |||||||||
| AB | 0.8172 | 0.7090 | 0.6670 | 0.7144 | 0.8646 | 0.7874 | 0.6788 | 0.5424 | |
| AC | 0.1025 | 0.3598 | 0.2416 | 0.7025 | 0.6808 | 0.4714 | 0.0742 | 0.6816 | |
| BC | 0.1626 | 0.8792 | 0.6668 | 0.1407 | 0.3979 | 0.6120 | 0.7311 | 0.3417 | |
| ABC | 0.7244 | 0.4239 | 0.7618 | 0.4665 | 0.9190 | 0.8705 | 0.3941 | 0.8460 | |
The seedlings were analysed 10 months after inoculation with Rhizopogon subareolatus and 5 months after the start of Mn stress. Cross sections were analysed by transmission electron microscopy coupled with energy-dispersive X-ray microanalyses (EDX). The concentrations obtained by EDX refer to volume units of the embedded specimen (nmol mm−3). n = 18–27 (±SE). Results of statistical analysis for cell-type-specific effects of mycorrhization and Mn stress are shown. Multivariate analysis of variance was conducted for cell type, inoculation with R. subareolatus and Mn stress as main effects in each variety separately. Two-sample comparison and t test was employed to compare the varieties.
Bold letters indicate significant P-values