Figure 3.

Cortical neurons overexpressing Par-4 show an increased accumulation of superoxide and hydrogen peroxide following mechanical injury compared to wild-type neurons. A. HE fluorescence (a measure of superoxide levels) and C. DCF fluorescence (a measure of hydrogen peroxide levels) were measured before injury and 12 hrs post injury in both wild-type (top) and Par-4 transgenic neurons (bottom). Fluorescence intensity of HE and DCF increases 12 hrs post injury in wild-type and Par-4 transgenic cortical neurons. B. Bar graph shows a higher accumulation of superoxide in neurons that overexpress Par-4 compared to wild-type neurons. D. Similar results are observed for the production of hydrogen peroxide in neurons overexpressing Par-4 when compared to wild-type neurons. Values are the means and standard derivations of determinations from eight separate cultures (20-30 neurons analyzed per culture). ***p<0.001 compared with corresponding values in cells from wild-type mice. ANOVA with Scheffe's post-hoc tests.