Table 1.
Primers and probes for real-time RT-PCR analyses of cytokines and YFV quantification in hamster tissues
| Cytokine | RT/Reverse primer (genome-sense) | Forward primer(mRNA-sense) | TaqMan probe (mRNA-sense) |
|---|---|---|---|
| INF-γ | CCGGAATCTTGGTGAGCTTCA | CCGGAATCTTGGTGAGCTTCA | TCCATGCTGCTGTTGAAG |
| IL-10 | AGCTGGACAACATACTACTCACTGA | GGTTTGGCAACCCAAGTAACC | CCTTACTGCAGGACTTTAA |
| TNF-α | CCGATCACCCCGAAGTTCAG | CCTCTTCTCCTTCCTGCTTGTG | CAGGCAGAAGAGGATTG |
| IL-12p40 | CGGGAATCAGAGGAGCTACTG | TGACTGCAATCAGCACTGACTT | TCTTGACGTTGAACTTC |
| TGF-β | CGGAGACGCCGAAGCA | GACACACAGTACAGTAAGGTCCTT | CTACAACCAACACAACCC |
| IL-2 | CATCTTCCAAGTGAAAGCTTTTGCT | CCAGTGCCTGGAAGAAGAACTT | TCCAGCACGCTCTGCAG |
| IL-4 | CCAGACGCCCTTTCAGCAA | CTGGGAAGCCCTGCAGAT | CACGGAGAAAGACCTC |
| β-actin | GGTGTGGTGCCAGATCTTCTC | CCCATTGAACACGGCATTGTC | ACCAACTGGGACGATATG |
| YFVNS3 | GAGCGACAGCCCCGATTTCT | AGGTCCAGTTGATCGCGGC | CAACGTCCAGACAAAAC |