Figure 2.

Simultaneous incubation with HGF fails to protect renal epithelial cells against oxidant stress-induced cell death. HKC-8 cells were treated simultaneously with or without 20 ng/mL HGF and 0.5 mM H₂O₂ for 16 hours in the absence of serum. A. HGF failed to promote cell survival after oxidant stress. B and C. HGF did not significantly protect HKC-8 cells from oxidant stress-induced cell death. Cell death was assessed by double staining with acridine orange and ethidium bromide. D. Simultaneous incubation of HKC-8 cells with HGF did not affect H₂O₂ -induced caspase-3 activation.