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HGF induces endogenous Akt and Bad phosphorylation in renal epithelial cells in the presence of oxidant stress. HKC-8 cells were treated with 20 ng/mL HGF for various periods of time as indicated, either simultaneously with or prior to addition of 0.5 mM H2O2. The cell lysates were immunoblotted with antibodies against phospho-Akt and phospho-Bad, or against total Akt and Bad protein, respectively. A. phospho-Akt (Ser473) and total Akt. B. phospho-Bad (Ser112) and total Bad.
