Figure 6.

Pre-administration of HGF gene prevents apoptosis after acute kidney injury induced by ischemia/reperfusion. Mice were administrated HGF expression plasmid (pCMV-HGF) or control vector (pcDNA3). Apoptosis was assessed in kidney cyrosections at day 2 after ischemia/reperfusion (I/R) by TUNEL staining. A-D. Representative micrographs demonstrated TUNEL-positive cells in various groups. E. Quantitative determination of apoptosis in the kidney after various treatments. *P < 0.05 verse sham controls. †P < 0.05 verse I/R controls (n = 4). Pre-admini., HGF gene was given 24 hours prior to I/R. Simul. Admini., HGF gene was given at the same time with I/R.