Table 1.
Immunohistochemical profiles
| Antibody | Manufacture | Clone | Dilution | Antigen retrieval | Tumor cells | Cyst-lining cells |
|---|---|---|---|---|---|---|
| Synaptophysin | Dako, CA | Sy38 | 1:200 | Citrate | +++ | ++ |
| Chromagranin | Chemicon, CA | LK2H10 | 1:4000 | Citrate | ++ | ++ |
| ER | Novacastra, MA | ER6F11 | 1:50 | Tris | ++ | ++ |
| PR | Neomarkers, CA | 1AR | 1:200 | Citrate | + | + |
| Pankeratin | * | * | * | * | +++ | +++ |
| CAM5.2 | BD Bioscience | Cam5.2 | 1:50 | P24 | +++ | +++ |
| GFAP | BD Bioscience | Polyclonal | 1:6000 | Citrate | − | − |
| Inhibin | Oxford Biochem, UK | RI | 1:50 | Citrate | − | − |
| Ki-67 | Dako, CA | MiB1 | 1:100 | Citrate | + | + |
| CDX2 | Biogenex, CA | CDX2-88 | 1:50 | Tris-EDTA | − | − |
| S100 | Biogenex, CA | A6 | 1:40 | No retrieval | − | − |
*
Keratin cocktail: AE1/AE3, DAKO, CA, 1:500; CAM5.2, Becton Dickinson, CA, 1:50; Cytokeratin MNF116, DAKO, CA, 1:50 and Keratin 8/18, Zymed, CA, 1:25