Table 1.
Primer sequences for (a) PCR and (b) real time PCR used in this study
| Primer | Sequence (5′–3′) | Product size (bp) | |
|---|---|---|---|
| (a) PCR | |||
| icaA forwarda | CAGTATAACAACATTCTATTG | 1,425 | |
| icaA reversea | GAGAATTGATAAGAGTTCC | ||
| icaC forwarda | ATAAACTTGAATTAGTGTATT | 1,017 | |
| icaC reversea | CCATAGCTTGAATAAGGG | ||
| IS256 forwardb | TGAAAAGCGAAGAGATTCAAAGC | 1,102 | |
| IS256 reverseb | ATGTAGGTCCATAAGAACGGC | ||
| lexA forward | GAACTCACTAAGCGACAAA | 592 | |
| lexA reverse | ATTACTTTACCTACTACAATGACA | ||
| Primer | Sequence (5′–3′) | Product size (bp) | AT (°C) |
|---|---|---|---|
| (b) Real time PCR | |||
| icaA-1 forward | GGAAGTTCTGATAATACTGCTG | 124 | 56 |
| icaA-1 reverse | GATGCTTGTTTGATTCCCTC | ||
| gyrB-3 forward | GGAGGTAAATTCGGAGGT | 129 | 57.1 |
| gyrB-3 reverse | CTTGATGATAAATCGTGCCA | ||
| recA-1 forward | AAAGTTCAGGTAAGACGACAG | 277 | 54 |
| recA-1 reverse | TCCCATTTCACCTTCAATTTCAG | ||