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Alexa Fluor 568 10 kDa dextran conjugate was injected into the insert membrane (injections A and C) and into the tissue culture (injection B). After applying the potential, injection B migrates toward the cathode, as shown in the sequence of images. Injections A and C remain in place despite the electric field. Intensities are measured at points 1–8 as a function of time. The smaller injections A and C are ∼135 μm in diameter. Injection B is ∼500 μm in diameter.
