TABLE 3.

The definitive ϕ-values and error bars for the α-spectrin SH3 domain (Spc-SH3), calculated from pH-scanning analysis

Mutation	ϕ-values (multipoint Leffler analysis)* pH-scanning	σϕ (multipoint Leffler analysis) pH-scanning	σϕ (two-point Leffler analysis) pH 7.0	σϕ (two-point Leffler analysis) pH 3.5	σϕ (two-point Leffler analysis) pH 2.5	σϕ (two-point Leffler analysis) AVERAGE
L8S	0.14 (4)	0.14	0.21	0.70	0.44	0.45
A11G	0.03 (3)	0.08	0.08	0.26		0.17
D14S	0.14 (4)	0.10	0.20		1.82	1.00
V23A	0.32 (3)	0.19	0.19	1.02		0.61
T24A	0.27 (4)	0.30	0.30	0.71	0.32	0.44
D29A	0.22 (4)	0.05	0.40	0.73	0.66	0.60
L33V	−0.28 (4)	0.21
S36N	0.26 (4)	0.32	0.94	1.10	0.65	0.90
K43A	0.24 (4)	0.27	0.32	1.08	0.42	0.61
V44A	0.50 (3)	0.12	0.09	0.31		0.20
V46A	1.20 (4)	0.21		0.58	0.16	0.37
N47G	0.40 (4)	0.34	1.05	1.60	0.45	1.03
D48G	1.27 (4)	0.44		2.35		2.35
F52A	0.61 (3)	0.19	0.12	0.28		0.20
V53A	0.72 (3)	0.69		0.26	0.54	0.40
A55G	0.61 (3)	0.41	0.24	0.30		0.27
V58A	0.18 (3)	0.16	0.21	0.36		0.28

^*Values in parentheses indicate the number of data points in the Leffler representation (Fig. 7).