FIGURE 5.

Prediction of the DCPA potentiation of early activation of NF-κB in Jurkat cells after stimulation with plate-bound anti-CD3 and anti-CD28. (a) NF-κB activation through the T cell receptor involves different membrane proximal signaling events compared to activation through the toll-like receptor 4 (TLR4) signaling pathway. These two signaling pathways act through TRAF6 to activate the IκB-NF-κB signaling module. (b) Nuclear NF-κB was assayed by NTFA at the indicated times after stimulation with plate-bound anti-CD3 and anti-CD28 and treatment with 0 μM (□) and 100 μM (+) DCPA. Two parameters (k1 and cN) of the mathematical model were calibrated to the ethanol control results, providing a prediction of the 100 μM DCPA results. The simulated time courses of active NF-κB without and pretreated with 100 μM DCPA are shown as solid and dashed curves, respectively. Each experiment was performed six times.