Figure 3.

Alternative splicing of exon 22 has little effect on PKC potentiation. (A) Fig. 1C replotted with receptor pairs connected by arrows to indicate effect of exchange of C2′ by C2. (B) Wild-type NR1₁₀₀ and NR1₁₀₁ and mutant NR1₁₀₀(ΔC2′) subunits. (C) Currents recorded from oocytes expressing mutant NR1₁₀₀(ΔC2′)/NR2 receptors before and after TPA treatment. (D) Mean potentiation of NR1₁₀₁/NR2A, NR1₁₀₀/NR2A, and NR1₁₀₀(ΔC2′)/NR2A receptors. In Ca²⁺, potentiation of NR1₁₀₁/NR2A, NR1₁₀₀/NR2A, and NR1₁₀₀(ΔC2′)/NR2A receptors was to 10.6 ± 0.7 (n = 9), 9.9 ± 0.6 (n = 24), and 11.8 ± 0.1.3 (n = 10) times control (differences not statistically significant). In Ba²⁺, potentiation was to 5.3 ± 0.0.7 (n = 9), 5.5 ± 0.5 (n = 19), and 10.8 ± 1.1 (n = 10) times control (***, P < 0.001 vs. each wild-type receptor).