Table 2. Angiogenic expression antibody profile.
| Antibody | Type of marker | Source | Concentration | Specificity and non-specific staining | Control sample |
|---|---|---|---|---|---|
| CD34 | Vascular | Novocastra NCL-END | 1 : 60 | Endothelium, connective tissue, erythrocytes | Tonsil |
| αSMA | Sigma A2547 | 1 : 20000 | Smooth muscle | Tonsil | |
| CD105 | Dako M3527 | 1 : 1000 | Proliferating endothelium, activated macrophages | Human breast carcinoma | |
| CD61 | Novocastra NCL-CD61-308 | 1 : 100 | Endothelium, platelets, megakaryocytes, monocytes, macrophages | Tonsil, human placenta | |
| VEGF | Neomarkers MS-1467 | 1 : 100 | 121,165, 189 Isoforms | Human breast carcinoma | |
| Ki-67 | Proliferation | Dako M7240 | 1 : 200 | 345, 395 Isoforms. nuclear expression | Tonsil |
| Glut-1 | Hypoxia | Dako A3536 | 1 : 200 | Erythrocytes, tumour | Tonsil |
| PDGF | Vascular/tumour/stromal | Novocastra NCL-PDEGF P-GF.44c | 1 : 60 | Tumour, macrophages, stroma, glia, epithelia | Tonsil, human placenta |
| Angiopoietin -1 | Santa Cruz Biotech Inc. H-98 (sc-8357) | 1 : 50 | Tumour, myocardium, vascular smooth muscle, lung, liver | Human placenta | |
| Angiopoietin -2 | Santa Cruz Biotech Inc. H-70 (sc-20718) | 1 : 50 | Tumour, ovary, placenta, uterus | Human placenta |
SMAα=α-smooth muscle actin.