Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1970 Aug;103(2):439–446. doi: 10.1128/jb.103.2.439-446.1970

Macroconidial Germination in Microsporum gypseum1

T J Leighton a,2, J J Stock a, R A Kelln a
PMCID: PMC248101  PMID: 5432010

Abstract

Biochemical events which occur during macroconidial germination have been studied in the dermatophyte Microsporum gypseum. The specific activity levels of various metabolic enzymes have been assayed during germination time periods. The accumulated levels of several of these enzymes, as a function of exogenous carbohydrate source, have been investigated. M. gypseum was found to possess a constitutive glyoxalate shunt, a constitutive glucokinase, a fructose phosphoenolpyruvate transferase, and a mannitol phosphoenolpyruvate transferase. The integration of endogenous reserve utilization during germination is discussed. The purification and properties of an alkaline phosphatase and its possible relationship to sporulation and spore germination also are described.

Full text

PDF
439

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barash I., Conway M. L., Howard D. H. Carbon catabolism and synthesis of macromolecules during spore germination of Microsporum gypseum. J Bacteriol. 1967 Feb;93(2):656–662. doi: 10.1128/jb.93.2.656-662.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bleyman M., Woese C. Ribosomal ribonucleic acid maturation during bacterial spore germination. J Bacteriol. 1969 Jan;97(1):27–31. doi: 10.1128/jb.97.1.27-31.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GEZELIUS K., WRIGHT B. E. ALKALINE PHOSPHATASE IN DICTYOSTELIUM DISCOIDEUM. J Gen Microbiol. 1965 Mar;38:309–327. doi: 10.1099/00221287-38-3-309. [DOI] [PubMed] [Google Scholar]
  4. HORIKOSHI K., IIDA S., IKEDA Y. MANNITOL AND MANNITOL DEHYDROGENASES IN CONIDIA OF ASPERGILLUS ORYZAE. J Bacteriol. 1965 Feb;89:326–330. doi: 10.1128/jb.89.2.326-330.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hanson T. E., Anderson R. L. Phosphoenolpyruvate-dependent formation of D-fructose 1-phosphate by a four-component phosphotransferase system. Proc Natl Acad Sci U S A. 1968 Sep;61(1):269–276. doi: 10.1073/pnas.61.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ikawa M., Borowski P. T., Chakravarti A. Choline and inositol distribution in algae and fungi. Appl Microbiol. 1968 Apr;16(4):620–623. doi: 10.1128/am.16.4.620-623.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Lee W. H. Carbon balance of a mannitol fermentation and the biosynthetic pathway. Appl Microbiol. 1967 Sep;15(5):1206–1210. doi: 10.1128/am.15.5.1206-1210.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leighton T. J., Stock J. J. Biochemical changes during fungal sporulation and spore germination. I. Phenyl methyl sulfonyl fluoride inhibition of macroconidial germination in Microsporum gypseum. J Bacteriol. 1970 Mar;101(3):931–940. doi: 10.1128/jb.101.3.931-940.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leighton T. J., Stock J. J. Heat-induced macroconidia germination in Microsporum gypseum. Appl Microbiol. 1969 Mar;17(3):473–475. doi: 10.1128/am.17.3.473-475.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lingappa B. T., Sussman A. S. Endogenous Substrates of Dormant, Activated and Germinating Ascospores of Neurospora Tetrasperma. Plant Physiol. 1959 Jul;34(4):466–472. doi: 10.1104/pp.34.4.466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Loomis W. F., Jr Developmental regulation of alkaline phosphatase in Dictyostelium discoideum. J Bacteriol. 1969 Oct;100(1):417–422. doi: 10.1128/jb.100.1.417-422.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morris D. L. Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent. Science. 1948 Mar 5;107(2775):254–255. doi: 10.1126/science.107.2775.254. [DOI] [PubMed] [Google Scholar]
  14. Ozaki H., Shiio I. Regulation of the TCA and glyoxylate cycles in Brevibacterium flavum. I. Ingibition of isocitrate lyase and isocitrate dehydrogenase by organic acids related to the TCA and glyoxylate cycles. J Biochem. 1968 Sep;64(3):355–363. doi: 10.1093/oxfordjournals.jbchem.a128902. [DOI] [PubMed] [Google Scholar]
  15. SLEIN M. W., CORI G. T., CORI C. F. A comparative study of hexokinase from yeast and animal tissues. J Biol Chem. 1950 Oct;186(2):763–780. [PubMed] [Google Scholar]
  16. Sjogren R. E., Romano A. H. Evidence for multiple forms of isocitrate lyase in Neurospora crassa. J Bacteriol. 1967 May;93(5):1638–1643. doi: 10.1128/jb.93.5.1638-1643.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Strandberg G. W. D-mannitol metabolism by Aspergillus candidus. J Bacteriol. 1969 Mar;97(3):1305–1309. doi: 10.1128/jb.97.3.1305-1309.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  19. de Vries W., Gerbrandy S. J., Stouthamer A. H. Carbohydrate metabolism in Bifidobacterium bifidum. Biochim Biophys Acta. 1967 Apr 25;136(3):415–425. doi: 10.1016/0304-4165(67)90001-3. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES