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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Nat Struct Mol Biol. 2008 May 30;15(6):573–580. doi: 10.1038/nsmb.1427

Figure 3.

Figure 3

Rpn1-Rpn2 stack extends the 20S proteolytic channel. (a) AFM images of: 20S CP (i), 20S–Rpn1 (ii), 20S–Rpn2 (iii) and 20S–Rpn2-Rpn1 (iv) and a separate mixture of Rpn1-Rpn2 (v). Dimensions of all particles are summarized in Supplementary Figure 1. Horizontal sections are provided for the leftmost complexes followed by a representative tilted enlargement, with a height bar (right). Multiparticle fields are shown in Supplementary Figure 3. (b) Statistical analysis of all samples based on height distribution identifies the species present in each mixture. A new population of extended 20S CP is found only in the presence of Rpn2 (iii) or Rpn1-Rpn2 (iv). An excess of starting reagents was always present is these mixtures.