Table 4.

Bacterial strains and plasmids

E. coli strains	Relevant characteristics	Reference
C122	prototroph, Δhsd	British Culture Collection Strain No. 122
BL21(DE3)	F- dcm, ompT hsdS (rB- mB-) gal λ (DE3)	[34]
DH10B	F- – mcrA Δ (mrr-hsdRMS-mcrBC) phi 80lacZDM15 Δ lacX74 recA1 endA1 ara D139 Δ(ara, leu)7697 galU galK rpsL nupG	Invitrogen
A0 34/86	serotype O83: K24: H31	[9,10]
DB24	dcm-6 dam-16::Kan mcr-62 Δ(mcrB-hsd-mrr)114	[33]
Plasmids
BAC	pBeloBAC11 cloning vector: ori2 (IncFI), designed for the construction of Bacterial Artificial Chromosomes (BACs), Cm R	[35]
BAC C4/1	about 56 kb insert from strain E. coli A0 34/86 (O83: K24: H31) into pBeloBAC11	[11]
pL series	series of pMECA plasmids containing lambda DNA fragments, ApR	[22]
pE series	E. coli K-12 chromosomal fragments subcloned into the pUC9 vector, ApR	[32]
pEco377I	pMECA plasmid with pEco377I recognition sequence at EcoRV site	[22]
pUC19	cloning vector, ApR	[36]
pGC1	hsdR, hsdM and hsdS genes of the EcoEI R-M system, ApR	[5]
pFFP30	hsd region of EcoAI on HindIII fragment cloned into pBR322, ApR	[5]
pJP24	HincII/NotI fragment from pJP21 carrying the hsdS of the EcoAI R-M system cloned into NdeI/NotI pET32a under control of the PT7g10 promoter. ApR	[37]
pJP21	A derivative of pFFP30 and pET32a carrying the hsdM and hsdS genes of the EcoAI R-M system under control of the PT7g10 promoter. ApR	[37]