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Tat broadens T cell responses against HIV-1 Gag. Mice were immunized with Gag alone, with Gag in combination with Tat protein, or with PBS alone. After 3 immunizations, fresh splenocytes were tested by IFNγ Elispot assay using 15 amino acid long peptides encompassing the entire Gag sequence. Positive responses to the indicated peptides are represented in the Figure.
