Table 1. Primer pairs and positions in the S10-spc-α locus of L. interrogans serovar Lai.
| Fragment | Primer pair | Position | Genes | Fragment | Primer pair | Position | Genes |
| 1. | 737-745 | 843-1435 | urp | 35.* | 301-258 | 11601-12581 | rplE-rplF |
| 2. | 740-744 | 1305-1873 | urp | 36.* | 301-191 | 11601-12948 | rplE-rplF |
| 3. | 748-751 | 1759-2493 | fus | 37. | 314-191 | 12348-12948 | rpsH, rplF |
| 4. * | 752-751 | 2269-2493 | fus | 38. | 314-428c | 12348-14047 | rpsH-rpmD |
| 5. * | 752-729 | 2269-2832 | fus | 39. | 314-430c | 12348-14372 | rpsH-rplO |
| 6. * | 735-729 | 2406-2832 | fus | 40. | 802-R1c | 12735-13445 | rplF, rplR |
| 7. * | 735-743 | 2406-3304 | fus | 41.* | 802-428c | 12735-14047 | rplF-rpmD |
| 8. | 735-667 | 2406-4394 | fus, tuf | 42.* | 802-430c | 12735-14372 | rplF-rplO |
| 9. * | 743c-706 | 3304-3814 | fus | 43.* | 191c-428c | 12948-14047 | rplF-rpmD |
| 10.* | 743c-667 | 3304-4394 | fus, tuf | 44. | 191c-430c | 12948-14372 | rplF-rplO |
| 11.* | 800-660 | 3683-4327 | fus, tuf | 45.* | R1-428c | 13427-14047 | rplR-rpmD |
| 12. | 800-667 | 3683-4394 | fus, tuf | 46. | R1-430c | 13427-14372 | rplR-rplO |
| 13.* | 657-654 | 4350-5255 | tuf | 47.* | 428-430c | 14047-14372 | rpmD, rplO |
| 14. | 657-624c | 4350-5976 | tuf-rplC | 48. | 428-G2c | 14047-15468 | rpmD-secY |
| 15. | 659-648 | 4438-5465 | tuf | 49.* | 430-G2c | 14372-15468 | rplO, secY |
| 16.* | 732-624c | 5240-5976 | tuf-rplC | 50 | 634-635 | 14643-16387 | rplO-adk |
| 17.* | 647-618 | 5297-5806 | tuf, rpsJ | 51.* | 443-G2c | 15276-15468 | secY |
| 18.* | 647-624c | 5297-5976 | tuf-rplC | 52.* | 443-G1 | 15276-15752 | secY |
| 19. | 624-650 | 5976-6790 | rplC, rplD | 53.** | SecYII-SecYIV | 15289-15946 | secY |
| 20.* | 624-644 | 5976-7151 | rplC-rplW | 54. | G2-G1 | 15468-15752 | secY |
| 21. | 624-621c | 5976-7847 | rplC-rplB | 55.* | G2-444 | 15468-15970 | secY |
| 22.* | 651-644 | 6883-7151 | rplD, rplW | 56. | G2-429 | 15468-16353 | secY-adK |
| 23.* | 643-621c | 7138-7847 | rplW, rplB | 57.* | G2-400 | 15468-16640 | secY-infA |
| 24.* | 622-621c | 7689-7847 | rplB | 58.* | 260-458c | 16616-18104 | infA-rpsD |
| 25. | 621-625 | 7847-8504 | rplB, rpsS | 59.* | 458-507 | 18104-18696 | rpsD, rpoA |
| 26.* | 621-605c | 7847-9082 | rplB-rpsC | 60.* | 458-504 | 18104-19376 | rpsD, rpoA |
| 27.* | 605-460 | 9082-10196 | rpsC-rpmC | 61. | 450-479 | 18163-19264 | rpsD, rpoA |
| 28.* | 801-803c | 10105-10965 | rpmC-rplX | 62.* | 477-504 | 18584-19376 | rpoA |
| 29. | 801-301c | 10105-11601 | rpmC-rplE | 63. | 477-501c | 18584-19791 | rpoA, rplQ |
| 30. | 801-300 | 10105-12110 | rpmC-rpsH | 64. | 503-480 | 18862-19621 | rpoA, rplQ |
| 31.* | 310-309 | 10167-10672 | rpmC-rplN | 65.* | 478-501c | 19371-19791 | rpoA, rplQ |
| 32.* | 310-277 | 10167-11107 | rpmC-rplX | 66.* | 478-502 | 19371-20341 | rpoA, rplQ, |
| 33.* | 310-301c | 10167-11601 | rpmC-rplE | 67. | 501-502 | 19791-20341 | rplQ |
| 34.* | 301-300 | 11601-12110 | rplE-rpsH |
*
Fragments used in the phylogenetic analysis from the binary data.
**
Primer pair used to produced G1–G2 sequences from all pathogenic species.