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. 2008 Jun 14;43(6):1018–1024. doi: 10.1016/j.jpedsurg.2008.02.025

Fig. 3.

Fig. 3

Rb phosphorylation at S807/811 and T826 is increased in adapting crypts. A, Crypts and villus enterocytes were isolated and verified by light microscopy. Protein was then extracted and analyzed by Western blot to further validate the isolation procedure by showing a crypt-specific proliferation marker PCNA and villus-distinctive marker PTK6. B, Western blot of protein expression of total/phosphorylated Rb, PCNA, and cyclin D1 in isolated crypts in mice after sham or SBR.