Figure 8. Schematic representation of IGF1 promoter fragments cloned into the pGL3 basic vector (A) and the corresponding luciferase activity (B) in human mesenchymal stem cells expressing EWS-FLI-1.

Restriction sites used for cloning are indicated. The exon 1 start codon, putative promoter regions GXP79580 and GXP641910 according to GEM analysis (Genomatix) are shown. Levels of luciferase activity, calculated as in Figure 6, are normalized to values obtained with the –1682 construct which was arbitrarily set to 100%. Error bars represent the S.E.M. of three independent experiments.