Figure 3.

The time course for receptor-mediated inhibition and the blocking of PIP₂ replenishment. Whole-cell K_ATP currents were activated by 100 μM pinacidil, which started at the times indicated by the arrows. (A) The inhibitory effects of 10 μM ACh before and after the application of 100 μM WMN. The applications of ACh and WMN are indicated by the bars above the recording. (B) The effect of intracellular dialysis with the pipette solution containing 4 mM AMPPNP. (C) The summary of recovery time courses after the washout of ACh. ●, Control; ◊, 10 μM WMN; □, 100 μM WMN; ▵, 4 mM AMPPNP in the pipette. At 4 min, the magnitudes of currents were 90.6 ± 6.6%, 46.0 ± 7.3%, 5.0 ± 0.2%, and 13.9 ± 7.9% of the control, respectively.