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Alu repeat plasmid constructs. (A) Alu repeat constructs used (arrows point to the 3′ ends of the Alu sequences) (20). (B) (Upper) Alu repeats cloned into the SmaI site (S) of the pSV2neo plasmid. The EcoRI (E), NcoI (N) fragment was analyzed by 2D electrophoresis. (Lower) Alu repeats cloned into the multiple cloning site of pYES2, between XhoI (X) and BamHI (B) sites. The BglI (Bg), BseRI (Bs) fragment was analyzed by 2D electrophoresis. Dashed lines indicate hybridization probes.
