Table 1.
List of selected genes differentially regulated after either Pieris brassicae oviposition or BC application
| Probe identification | Gene symbol | AGI code | Eggs | P value | BC | P value |
|---|---|---|---|---|---|---|
| Induced genes | ||||||
| Cell wall metabolism | ||||||
| Expansin 15 | EXPA15 | At2g03090 | 1.56 | 0.014 | 0.96 | 0.500 |
| Xyloglucan endotransglycosylase 6 | XTH6 | At5g65730 | 1.76 | 0.025 | 1.56 | 0.045 |
| Defense | ||||||
| Plant defensin 1.2b | PDF1.2b | At2g26020 | 1.22 | 0.481 | 1.66 | 0.049 |
| Tryptophan synthase, subunit 1 | TSA1 | At3g54640 | 0.97 | 0.946 | 1.62 | 0.044 |
| Protein metabolism/ metabolism | ||||||
| Eukaryotic translation initiation factor 3 subunit H1 | TIF3H1 | At1g10840 | 1.96 | 0.011 | 2.11 | 0.019 |
| Gamma-glutamyl hydrolase 2 | GGH2 | At1g78680 | 2.06 | 0.040 | 3.22 | 0.011 |
| Cyclophilin 5 | CYP5 | At2g29960 | 1.81 | 0.012 | 0.86 | |
| Ribosomal protein large subunit 16A | RPL16A | At2g42740 | 1.77 | 0.043 | † | |
| MMS 2 homolog 4 | MMZ4 | At3g52560 | 2.18 | 0.007 | 2.30 | 0.032 |
| Clp amino terminal domain-containing protein | At4g12060 | 1.72 | 0.032 | 0.80 | ||
| Aldehyde dehydrogenase 3F1 | ALDH3F1 | At4g36250 | 2.29 | 0.016 | † | |
| 14–3-3 protein GF14 λ | GRF6 | At5g10450 | 1.65 | 0.005 | 2.39 | 0.036 |
| Acetolactate synthase small subunit | At5g16290 | 1.65 | 0.021 | 1.28 | 0.791 | |
| Protein kinase | At5g50000 | 2.12 | 0.022 | 0.96 | * | |
| Transport | ||||||
| MATE efflux family protein | At1g73700 | 1.77 | 0.001 | 1.52 | 0.065 | |
| Pleitropic drug resistance 3 | PRD3 | At2g29940 | 1.65 | 0.001 | 1.37 | 0.031 |
| Metal transporter | At3g08650 | 1.92 | 0.030 | 1.43 | * | |
| Pleitropic drug resistance 1 | PDR1 | At3g16340 | 1.62 | 0.011 | 1.27 | 0.480 |
| Other processes | ||||||
| 50S ribosomal protein L21 | RPL21 | At1g35680 | 1.85 | 0.014 | 1.91 | * |
| Dehydrin | At1g54410 | 1.77 | 0.035 | 1.00 | 0.999 | |
| Delta 7-sterol-C5-desaturase | At3g02590 | 1.57 | 0.044 | 1.48 | 0.382 | |
| Telomerase activator 1 | TAC1 | At3g09290 | 2.13 | 0.017 | 2.24 | 0.097 |
| Cardiolipin synthase | CLS | At4g04870 | 2.59 | 0.021 | 2.89 | 0.012 |
| Chlorina 42 | CHLI1 | At4g18480 | 1.78 | 0.019 | 1.22 | 0.518 |
| Prenylcysteine alpha-carboxyl methyltransferase | STE14B | At5g08335 | 2.63 | 0.043 | 0.98 | * |
| hydrolase, α/β fold | At5g16120 | 2.46 | 0.002 | 0.98 | * | |
| Repressed genes | ||||||
| Cell structure metabolism | ||||||
| GDSL-motif lipase/hydrolase | At1g29660 | 1.26 | 0.246 | 0.66 | 0.041 | |
| Kinesin 13A | At3g16630 | 0.77 | 0.082 | 0.66 | 0.041 | |
| Proline-rich extensin-like protein | At4g08370 | 1.08 | 0.802 | 0.53 | 0.033 | |
| Proline-rich extensin-like protein | At4g08400 | 1.09 | 0.751 | 0.58 | 0.004 | |
| Phosphatidate cytidylyltransferase | At4g26770 | 0.74 | 0.497 | 0.57 | 0.003 | |
| BCL-2-associated athanogene 1 | BAG1 | At5g52060 | 0.77 | 0.474 | 0.66 | 0.009 |
| Defense | ||||||
| Peroxisome defective 1 | PED1 | At2g33150 | 0.56 | 0.029 | 0.80 | 0.249 |
| receptor-like protein kinase | At3g45860 | 0.61 | 0.030 | 0.64 | 0.099 | |
| Protein metabolism / metabolism | ||||||
| Ribosomal protein L4 | RPL4 | At1g07320 | 0.67 | 0.002 | 0.81 | * |
| Cysteine proteinase | At1g29110 | 0.63 | 0.035 | 0.84 | 0.123 | |
| RNA binding protein | At5g12280 | 0.63 | 0.007 | 0.73 | 0.203 | |
| Protein kinase | At5g41260 | 0.61 | 0.038 | 0.73 | * | |
| Transport | ||||||
| Amino acid permease | At5g01240 | 0.54 | 0.025 | 0.72 | 0.405 | |
| Mitochondrial substrate carrier family protein | At5g15640 | 0.52 | 0.015 | 0.89 | 0.441 | |
| Copper-binding family protein | At5g24580 | 0.66 | 0.035 | 0.86 | 0.069 | |
| Other processes | ||||||
| bZIP transcription factor | At1g19490 | 0.63 | 0.048 | † | ||
| One-helix protein 2 | OHP2 | At1g34000 | 0.67 | 0.012 | 0.82 | 0.194 |
| Quinolinate phophoribosyltransferase | QPT | At2g01350 | 0.53 | 0.004 | 0.49 | 0.021 |
| Hydrolase | At2g27500 | 0.66 | 0.040 | 0.81 | 0.042 | |
| Cytochrome P450 | CYP705A21 | At3g20120 | 0.65 | 0.041 | 0.69 | 0.036 |
| Phospholipase C 1 | PLC1 | At4g38530 | 0.67 | 0.010 | † | |
Relative changes in gene expression 72 h after egg deposition or BC application in Brussels sprout plants. Mean expression ratios are calculated from three biologically independent replicates. Only genes for which information about their possible function in Arabidopsis is present are shown.
*70-mer oligonucleotide hybridized only in one replicate.
†70-mer oligonucleotide did not hybridize in any of the three replicates. AGI, Arabidopsis Genome Initiative.