Fig. 3.

Phylogenetic analysis of insertion-site sequences. Shown are unrooted phylogenetic trees based on multiple alignments of sequences flanking both sides of the Tc1 insertion point. Trees are shown in Phylip format, with bootstrap values indicated. Data in A are based on “left”-flanking sequences (centromeric side of insertion), and data in B are based on right-flanking sequences (telomeric side of insertion). Segment lengths refer to the non-Medea GA2 (USA) strain. Countries of origin include Brazil, Uganda, Australia, India, Japan, the United States, Canada, Nigeria, Costa Rica, the Philippines, Colombia, Ivory Coast, Singapore, Thailand, and Peoples' Republic of China, abbreviated BRZ, UGN, AUS, IND, JPN, USA, CAN, NIG, COS, PHL, COL, IVC, SNG, THL, and PRC, respectively. UNK was collected by Alexander Sokoloff before 1970 and is of unknown origin. The USA (GA2) strain, which was the source of the whole-genome sequence, was collected in 1983. All other strains were collected in 1988. Brackets to the right indicate grouping of M¹ (“M”) and non-M¹ (“+”) strains, as determined by genetic testing (see Materials and Methods).