Fig. 2.

Altered H/ACA RNA levels and delayed rRNA processing in Dkc1^Δ15 ES cells. (A) Decreased levels of specific H/ACA RNA Dkc1^Δ15 ES cells as assessed by Northern blot analysis. Five micrograms of total RNA from WT and Dkc1^Δ15 ES cells was loaded in each lane and hybridized with ³²P-labeled mouse H/ACA RNA probes. The C/D box RNA mU14 was used as a loading control. (B) Pulse–chase labeling experiments of rRNA isolated from WT and Dkc1^Δ15 ES cells. Cells were labeled with l-[³H-methyl]methionine for 30 min and then chased in nonradioactive medium for the times shown. The RNA was separated on a 1.25% agarose gel, transferred to a nylon filter, and exposed to x-ray film.