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. 2008 Jul 9;105(29):10209–10214. doi: 10.1073/pnas.0712144105

Fig. 4.

Fig. 4.

RSV uses a Vps4-independent budding mechanism. Cells (293T) transfected with Vps4a constructs: pEGFP vector (EGFP) or Vps4a-EGFP (Vps4a), or dominant-negative mutants Vps4a-K173Q-EGFP (K173Q) or Vps4a-E228Q-EGFP (E228Q) (A), or Vps4b constructs: pDsRed vector (DsRed) or Vps4b-DsRed (Vps4b), or dominant-negative mutants Vps4b-K180Q-DsRed (K180Q) or Vps4a-E235Q-DsRed (E235Q) (B). Cells were infected 24 h after transfection at a moi of 1.0 with either WT RSV or HIVNL4−3. Supernatant virus was collected 48 h after infection and assayed for either RSV by viral titer (gray bars) or HIV p24 by ELISA (black bars).