Fig. 5.

FIP2 associates with both viral inclusions and viral filaments. (A–P) MDCK cells expressing FIP2-GFP variants [FIP2-WT (A–D), FIP2-ΔC2 (E–H), or FIP2-ΔRBD (I–L)] infected with RSV or MDCK cells infected with recombinant RSV expressing GFP (M–P) were stained by indirect immunofluorescence for both RSV F (B, F, J, and N) and RSV N (C, G, K, and O). In the overlays (D, H, L, and P), FIP2-GFP variants and GFP are pseudocolored green, RSV F is pseudocolored red, and RSV N is pseudocolored blue. The arrows with tails indicate viral inclusion body location, whereas the arrows without tails indicate an area of dense viral filament formation. (Q and R) Percentage of the total integrated pixel intensity indicating colocalization of FIP2-GFP variants or GFP with RSV F (Q) or RSV N (R) were calculated. The median value is shown. ***, P < 0.0001; **, P ≤ 0.009; Wilcoxon rank sum test. No significant difference was measured among WT, ΔC2, and GFP groups in graph Q. Additional comparisons on graph R demonstrate that the WT differs significantly from ΔC2 and GFP (both P < 0.0001); ΔRBD differed significantly from GFP (P < 0.0001).