Skip to main content
. 2008 Jul 16;105(29):9959–9964. doi: 10.1073/pnas.0804336105

Fig. 5.

Fig. 5.

Identification of reversibly oxidized PTPs in PDGF-BB-transformed angiomyolipoma cells by using IAP- and BBP-based-probes. Serum-deprived (16 h) angiomyolipoma cell lines (lanes: 1, SV7tert; 2, SV7tert-PDGF; 3, SV7tertPDGF-Tumor1; 4, SV7tertPDGF-Tumor2) were subjected to the cysteinyl-labeling assay using either biotinylated IAP (A–D) or BBP probes (E and F). Biotinylated proteins were purified on streptavidin–Sepharose beads, resolved by SDS/PAGE, and immunoblotted against SHP2 (A and E), PTEN (B and F), MKP-1 (C and G), or LAR (D and H). Reversible PTP oxidation was restricted to PDGF-BB-transformed angiomyolipoma cells using both probes. WB, Western blotting.