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RPTPα reversible oxidation is detected by using the cysteinyl-labeling assay. Serum-deprived (16 h) angiomyolipoma SV7tert cells were exposed to 200 μM H2O2 for 1, 5, or 10 min and subjected to the cysteinyl-labeling assay using a biotinylated IAP probe. Biotinylated proteins were purified on streptavidin–Sepharose beads, resolved by SDS/PAGE, and immunoblotted by using anti-RPTPα antiserum.
