Table 1.
Kinetic Constantsa for Transport of Antimicrobial Agents by Gingival Fibroblasts
| Agent | Km | Vmax | |
|---|---|---|---|
| μM | (μg/ml) | ng/min/μg protein | |
| Minocycline | 219 ± 14.0 | (108 ± 6.90) | 14.2 ± 0.77 |
| Doxycycline | 432 ± 29.3 | (208 ± 14.1) | 2.48 ± 0.22 |
| Tetracycline | 293 ± 23.9 | (141 ± 11.5) | 1.79 ± 0.18 |
| Ciprofloxacin | 628 ± 43.5 | (200 ± 14.4) | 4.62 ± 0.31 |
| Ofloxacin | 634 ± 56.8 | (229 ± 20.5) | 4.02 ± 0.21 |
| Moxifloxacin | 457 ± 37.9 | (200 ± 16.6) | 8.57 ± 0.67 |
a
Determined by Lineweaver-Burk analysis of transport activity assayed during the rapid initial phase of uptake (3 minutes). Results are expressed as mean ± SEM of at least 5 experiments