(A) FLAG-tagged MAGP-2 protein was expressed in bacterial cells and isolated by anti-FLAG chromatography. MAGP-2 preparations were confirmed by comassie staining (Co), and by western blot analysis with either anti-MAGP-2 antibodies (M) or anti-FLAG M2 antibodies (F). The arrow denotes MAGP-2 protein. (B) MB114 and HUVEC cells were transiently transfected with pHes1-luciferase plus pCMV-β-gal and subsequently treated with rMAGP-2 (1 to 5 μg/ml). Afterward, luciferase and β-gal activities contained in detergent-solubilized cell extracts were measured. Data are mean (± SEM) of 2 independent experiments. (C) GFP- and MAGP-2-expressing MB114 cells were transiently transfected with pHes1-luciferase and pCMV-β-gal cDNAs, together with or without Jagged-1 cDNA as indicated. Afterward, luciferase and β-gal activities were measured as above. Data are the mean (± SEM) of four independent experiments. (*, **, ***, p < 0.05; Student’s Test).