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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Microvasc Res. 2008 Mar 12;76(1):7–14. doi: 10.1016/j.mvr.2008.01.001

Figure 2. MAGP-2 inhibits Notch1 receptor processing.

Figure 2

(A) 293T cells were transiently transfected with cDNAs encoding Myc-tagged versions of Notch1, Jagged-1, and MAGP-2 in various combinations as indicated. Accumulation of N1ICD was monitored by immunoblotting whole cell extracts with monoclonal antibodies (α-Val1744) that only recognize the N1ICD domain. Afterward, the blot was stripped and immunoblotted with anti-myc monoclonal antibodies to control for differences in Notch1 and Jagged1 expression or with anti-β-actin monoclonal antibodies to control differences in protein loading. Shown is a representative experiment that was performed three times in its entirety. (B) Human 293T cells were transfected with Hes-1 luciferase and CMV-β-gal reporters either alone (B=basal) or in addition to Notch1 (N), Notch1 + Jagged1 (NJ), or Notch1 + Jagged1, + MAGP-2 (NJM) as described above. Empty vector was used to normalize total DNA transfected in all wells. Afterward, luciferase and β-gal activities were measured as described (materials and methods). Data are the mean (± SEM) of three independent experiments. (*, **, p < 0.05; Student’s Test).