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. 2008 Aug 6;3(8):e2849. doi: 10.1371/journal.pone.0002849

Figure 4. Some mRNA species are more susceptible to oxidative damage in SOD1G93A mice.

Figure 4

(A) Semi-quantitative RT-PCR analysis confirmed that the identified mRNA species by DNA microarray were present in the oxidized mRNA pool. MBP, cytochrome c, cytochrome c oxidase Va and ribosome protein S6 mRNAs, which had strong signal intensities on the arrays, were present in the oxidized mRNA pool. MAP2 and PCM1 mRNAs, which had very weak signal intensities on the arrays, were hardly detected in the oxidized mRNA pool. These oxidized mRNA species are significantly decreased in vitamin E treated SOD1G93A mice (G93A+vitE). (B) Semi-quantitative RT-PCR analysis showed that the oxidized mRNA species are not upregulated in the whole spinal cord of SOD1G93A mice compared to their non-transgenic littermates. SOD1 mRNA, which includes endogenous mouse SOD1 and transgenic human SOD1, was used as a control. n = 3.